Origin of Replication Options

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Bacterial Origin Cloning: We currently have four alternative origins of replication for use in bacteria. Our bacterial origins of replication are normally flanked by two SwaI restriction sites. They will function if they are inserted in either orientation between these two sites.

Phage and Mammalian Origin Cloning: We also have a range of other origins of replication, including the SV40 origin of replication for mammalian cells and the F1 phage origin of replication that can be used to create single stranded DNA. These are currently available in either the SbfI or PacI sites respectively, however, they can be inserted into other positions on request.

Bacterial Origin Products

pSF-CMV-pUC - pUC origin (Highest Copy)
pSF-CMV-BR322 - BR322 Origin (15-20 copies per cell)
pSF-CMV-p15A - p15A Origin (~10 copies per cell)
pSF-CMV-SC101 - SC101 Origin (~5 copies per cell)

Phage Origin Products

pSF-CMV-F1 Ori PacI

Mammalian Origin Products

pSF-CMV-SV40 Ori SbfI
pSF-CMV-Ub-Hygro-SV40 Ori SbfI
pSF-CMV-Ub-Puro-SV40 Ori SbfI
pSF-CMV-Ub-Zeo-SV40 Ori SbfI
pSF-CMV-Ub-Neo/G418-SV40 Ori SbfI